Wilko recently announced 400 employees layoff from the company.
The retailer, which has stores in Cheltenham, Gloucester, and Stroud, plans to cut hours for team supervisors in 150 of its 401 stores, the equivalent of about 150 full-time equivalent job losses, after a fall in sales.
The cuts also include about 150 assistant store managers and around 95 workers from its Nottinghamshire contact center. Wilko said it is supporting affected individuals.
The retailer said the proposed changes to its management structure will help the business to stabilize and thrive.